General Microbiology

In a Word document, use the prompts below to conduct research and answer each of the questions based on this week’s experiments. Remember to use full sentences and proper citations for each of your answers. References section required at the end of assignment.


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  1. In your student research project, you will use Bergey’s manual to identify unknown bacteria. Briefly mention the correct order of steps where we tried to get information about our unknown from previous experiments starting student research project in this semester. (4 points)


  1. Compare your API test kit result to your Bergey’s manual result. Which test do you think is more accurate? Why or why not? (4 points)


  1. What two groups of Gram negative bacteria can be differentiated with the Oxidase test results? Why? (2 points)


  1. You are trying to identify a known Gram positive bacterial species Mycobacterium smegmatis. With the help of a flow chart in Bergey’s Manual dichotomous key shown below, can you describe the key biochemical tests used to identify Mycobacterium smegmatis? (2 points)
  2. You’ve been asked to create a growth medium used to isolate a Vibrio species from a mixed culture of Aeromonas, Pseudomonas, and Vibrio. Given the abbreviated dichotomous key shown below, what two key elements must you include to best ensure a medium selective for Vibrio? (2 point)



Answer questions 6 and 7 based on the following prompt:

After several differential tests, it was concluded that unknown #29 was Escherichia coli. After performing the Gram stain to determine that the unknown was a Gram negative rod, the organism was grown on a TSA slant for use in inoculating the rest of the biochemical tests. All of the biochemical tests worked well, except for the Indole test. You propose that the result was possibly a false negative. This was determined since it was inconsistent with the rest of the results. The TA suggested that the test be repeated. The repeated test gave a positive result. 


  1. Is this new positive result consistent with the other data? How can you conclude that the unknown is Escherichia coli using Bergey’s manual?(2 points)


  1. How did the test results lead to identification? What problems were encountered? (2 points)


Grammar: 1 point

In-text citations and references: 1 point

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